Chromatography

Background Chlorophyll often hides the a nonher(prenominal) pigments present in leaves. In autumn, chlorophyll breaks down, allowing xanthophyll and carotene, and newly made anthocyanin, to carry their colors. The modify of pigments in a folio may be dislocated into bands of color by the proficiency of topic chromatography. Chromatography involves the separation of mixtures into person components. Chromatography means color writing. With this technique the components of a mixture in a liquid intermediate are spaced. The separation takes train by absorption and capillary action. The motif holds the substances by absorption; capillarity pulls the substances up the story at different rates. Pigments are divide on the make-up and show up as good-for-nothing streaks. The pattern of separated components on the theme is called a chromatogram. Question Which pigments tush you identify in a green leaf? Prediction The pigments that shoot on on the chromatograph report should be related to pigments seen on plants in real life. out of various possibilities, I study that trine pigments that may be discovered in this science lab allow be carotene, chlorophyll a, and xanthrophylls. Safety Precautions The solvent is volatile. discover that in that respect are no flames in the classroom, and empty breathing vapors from the solvent.

The classroom must be well-ventilated Materials * prickly-seeded spinach plant leaves * Solvent (90% rock anoint ether and 10% acetone) * Chromatography paper * opus clip * Retort stand * Test-tube clamp * Cork stopper * go after glass * Large severalize tube Procedure 1. Attach the sizeable test tube to a retort stand. 2. Set up the bobsleigh stopper and the paper clip as shown. 3. saloon a piece of chromatography paper so that it is pine comme il faut to go down from the paper clip but not so long that it touches the tin of the test tube. (Refer to the diagrams). Cut the paper to a advert at one remove. 4. Place a coleus or spinach leaf over the pointed end of the chromatography...If you want to get a full essay, order it on our website: Orderessay

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